ER stress-activated indicator (ERAI), ERAI mice ER stress-activated indicator (ERAI), ERAI mice <A HREF="https://mus.brc.riken.jp/en/mouse_of_month/mar_2007_mm" target="_blank">Mouse of the Month Mar 2007</A><br><a href="https://mus.brc.riken.jp/ja/wp-content/uploads/pdf/blc/01099_GB.pdf">Genetic Background</a> ヘテロ型である場合は野生型C57BL/6と同様に飼育・交配が可能である。ホモ型の表現型は未確認。 Developed by Takao Iwawaki and Masayuki Miura, RIKEN Brain Science Institute. The 3.6-kb fragment of pCAX-F-XBP1deltaDBD-venus was injected into the pronuclei of C57BL/6 fertilized eggs. Transgenic mice were backcrossed to C57BL/6JJcl. B (1-3 months) 条件を付加する。利用者は事前に寄託者の提供承諾書を得る。<br>研究成果の公表にあたって寄託者の指定する文献を引用する。Nat. Med., 10, 98-102 (2004).<br>研究成果の公表にあたって謝辞の表明を必要とする。<br>寄託後２年間は共同研究として用い、論文や学会での発表の際は共著とする。寄託者の研究と競合する用途を避けるために、予定の研究概要を寄託者に知らせ、使用許可を得る。利用は非商業的研究・教育目的とする。もし商業的利用をする場合は理化学研究所と協議する。本試料は理化学研究所の成果であることを明記する。 開発年：2002年 開発者：岩脇隆夫先生、三浦正幸先生 機関名：理研BSI 東京大学 Univ. Tokyo ヒトXBP1遺伝子とvenus (改良型GFP) 遺伝子との融合遺伝子をトランスジーンとして作製したトランスジェニックマウスで、小胞体ストレスを受けた組織は蛍光を発する。 C57BL/6J-Tg(CAG-XBP1*/venus)#Miur/MiurRbrc ER stress-activated indicator (ERAI) ER stress-activated indicator (ERAI) Cell Biology Research Necessary documents for ordering:<ol><li>Approval form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx">English</A>)</li><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the Venus using Approval form (<a HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/Form_V.docx">Form V (English)</a>) (Lab Contact: Laboratory for Cell Function Dynamics, RIKEN CBS: mta-cfds@ml.riken.jp).</li><li>CAGGS MTA (<A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/CAGGS_MTA.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol><A HREF="https://idenut.f.u-tokyo.ac.jp/index.html" target="_blank">Lab HP (Japanese)</A> ERAI (ER stress-activated indicator) transgenic mice carry human XBP1 and venus (a variant of green fluorescent protein) fusion gene under control of a CAG promoter. Endoplasmic reticulum (ER) stress is caused by the accumulation of unfolded proteins in the ER lumen, and is associated with vascular and neurodegenerative diseases. The transcripts from ERAI constructs are spliced under ER stress, the spliced mRNA is translated into an XBP1-venus fusion protein, which can be detected by fluorescence of the venus. This strain is useful to monitor physiological and pathological ER stress in vivo. 開発年：2002年開発者：岩脇隆夫先生、三浦正幸先生機関名：理研BSI Carrier x Noncarrier [C57BL/6JJcl]. Tg mice are viable and fertile. true ERAI mice 三浦　正幸 B（1〜3か月） Fluorescent Proteins/lacZ System Masayuki MIURA <a href='https://brc.riken.jp/mus/pcr01099'>Genotyping protocol -PCR-</a> The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Med., 10, 98-102 (2004).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. During 2 years after deposition by the DEPOSITOR to the RIKEN BRC, the RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR, and to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE and must obtain a prior permission from the DEPOSITOR to avoid the conflict of interest with the DEPOSITOR. The material shall be used for teaching or not-for-profit research purposes only for the research described within this MTA. If there is intent to use the material for profit purpose, the recipient shall first discuss its intentions with RIKEN. The recipient shall agree to acknowledge RIKEN in all publications with regard to the material. CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), SV40 intron and polyA signal, human XBP1 cDNA, Jellyfish venus (GFP) cDNA, FLAG tag RBRC01099